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验证数据展示

  • WB analysis using 32034-1-AP

    WB analysis using 32034-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32034-1-AP (FAM162A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 32034-1-AP

    IHC staining of mouse colon using 32034-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 32034-1-AP (FAM162A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 32034-1-AP

    IF Staining of A431 using 32034-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FAM162A antibody (32034-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

FAM162A Polyclonal antibody

FAM162A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32034-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

C3orf28, DC16, E2IG5, E2-induced gene 5 protein, FWP001

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCaki-2 cells, HepG2 cells, RT-4 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32034-1-AP targets FAM162A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36569

Product name: Recombinant human FAM162A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-154 aa of BC015060

Sequence: MGSLSGLRLAAGSCFRLCERDVSSSLRLTRSSDLKRINGFCTKPQESPGVPSRTYNRVPLHKPTDWQKKILIWSGRFKKEDEIPETVSLEMLDAAKNKMRVKISYLMIALTVVGCIFMVIEGKKAAQRHETLTSLNLEKKARLKEEAAMKAKTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 162, member A
别名 C3orf28, DC16, E2IG5, E2-induced gene 5 protein, FWP001
计算分子量 154 aa, 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC015060
基因名称 FAM162A
Gene ID (NCBI) 26355
RRIDAB_3670175
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96A26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Family with sequence similarity 162 member A (FAM162A, also known as E2IG5, HGTD-P, and C3orf28) is a mitochondrial apoptotic protein, which is known as an HIF-1 alpha-responsive pro-apoptotic molecule (PMID: 15082785; 16698020; 22776087). When FAM162A was overexpressed, the hypoxia signal was sent directly to the mitochondria and caused cell death (PMID: 16698020).

实验方案

Product Specific Protocols
IF protocol for FAM162A antibody 32034-1-APDownload protocol
IHC protocol for FAM162A antibody 32034-1-APDownload protocol
WB protocol for FAM162A antibody 32034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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