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FAM134B Polyclonal antibody
FAM134B Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, HEK-293 cells, rat brain tissue, mouse brain, mouse cerebellum |
| Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21537-1-AP targets FAM134B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | IF, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | FAM134B fusion protein Ag16176 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | family with sequence similarity 134, member B |
| Synonyms | FAM134B, JK-1, Protein FAM134B, Reticulophagy receptor 1, RETREG1 |
| Calculated Molecular Weight | 497 aa, 55 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC030517 |
| Gene Symbol | FAM134B |
| Gene ID (NCBI) | 54463 |
| RRID | AB_2878879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6L5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM134B, also known as the reticulophagy regulator 1 (RETREG1) or JK-1, is a member of the family with sequence similarity 134. FAM134B is an important ER-phagy receptor. FAM134B regulates the size and shape of the ER and participates in some ER-phagy-related processes(PMID: 33199694). FAM134B inhibition contributes to impair proteostasis in the ER due to the accumulation of misfolded or aggregated proteins, which in turn leads to compromised neuronal survival and progressive neuronal degenerative diseases. In addition, FAM134B mutations are common in patients with colorectal adenocarcinoma, and oesophageal squamous cell carcinoma(PMID: 29226326).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM134B antibody 21537-1-AP | Download protocol |
| IHC protocol for FAM134B antibody 21537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Cells Deploy a Two-Pronged Strategy to Rectify Misfolded Proinsulin Aggregates.
| ||
Nat Commun Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD. | ||
Dev Cell Calnexin controls TrkB cell surface transport and ER-phagy in mouse cerebral cortex development | ||
Autophagy Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy. | ||
