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FAM134A Polyclonal antibody

FAM134A Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24650-1-AP

Print datasheet

Synonyms

C2orf17, FAM134A, Protein FAM134A

验证数据展示

  • WB analysis using 24650-1-AP

    WB analysis using 24650-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24650-1-AP (FAM134A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 24650-1-AP

    IHC staining of mouse brain using 24650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24650-1-AP

    IHC staining of mouse brain using 24650-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24650-1-AP (FAM134A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 24650-1-AP

    IF Staining of U2OS using 24650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FAM134A antibody (24650-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inmouse thymus tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24650-1-AP targets FAM134A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen FAM134A fusion protein Ag20025 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 134, member A
Synonyms C2orf17, FAM134A, Protein FAM134A
Calculated Molecular Weight 543 aa, 58 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC002420
Gene Symbol FAM134A
Gene ID (NCBI) 79137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NC44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein family with sequence similarity 134 (FAM134) consists of three proteins, FAM134B, FAM134A, and FAM134C, which share the same LIR amino acid sequence. FAM134 reticulon family proteins (FAM134A, FAM134B, and FAM134C) are ER-phagy receptors with structural similarities and nonredundant functions. The protein family with sequence similarity 134 (FAM134). Fam134A (also known as metastasis-associated genes 2, MAG-2) is widely expressed in various types of tissues including tumors, and is considered to be closely correlated with tumor proliferation, invasion, and metastasis.

实验方案

Product Specific Protocols
WB protocol for FAM134A antibody 24650-1-APDownload protocol
IHC protocol for FAM134A antibody 24650-1-APDownload protocol
IF protocol for FAM134A antibody 24650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols