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FAM129B Polyclonal antibody

FAM129B Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22553-1-AP

Print datasheet

Synonyms

C9orf88, FAM129B, MEG 3, Niban like protein 1, OC58, Protein FAM129B

验证数据展示

  • WB analysis of NIH/3T3 using 22553-1-AP

    WB analysis of NIH/3T3 using 22553-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22553-1-AP (FAM129B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 22553-1-AP

    FC experiment of HeLa using 22553-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human FAM129B (22553-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

经过测试的应用

Positive WB detected inNIH/3T3 cells, A431 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22553-1-AP targets FAM129B in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen FAM129B fusion protein Ag18314 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 129, member B
Synonyms C9orf88, FAM129B, MEG 3, Niban like protein 1, OC58, Protein FAM129B
Calculated Molecular Weight 733 aa, 83 kDa
Observed Molecular Weight 83-93 kDa
GenBank Accession NumberBC067366
Gene Symbol FAM129B
Gene ID (NCBI) 64855
RRIDAB_2879121
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96TA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM129B, also named as Niban-like protein 1 or C9orf88, is a 746 amino acid protein, which contains one PH domain and belongs to the Niban family. FAM129B localizes in the cytoplasm and may play a role in apoptosis suppression and promote melanoma cell invasion in vitro.

实验方案

Product Specific Protocols
WB protocol for FAM129B antibody 22553-1-APDownload protocol
IHC protocol for FAM129B antibody 22553-1-APDownload protocol
FC protocol for FAM129B antibody 22553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols