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验证数据展示

  • WB analysis of NIH/3T3 using 22553-1-AP

    WB analysis of NIH/3T3 using 22553-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22553-1-AP (FAM129B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM129B Polyclonal antibody

FAM129B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22553-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C9orf88, FAM129B, MEG 3, Niban like protein 1, OC58, Protein FAM129B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A431 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22553-1-AP targets FAM129B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 FAM129B fusion protein Ag18314 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 129, member B
别名 C9orf88, FAM129B, MEG 3, Niban like protein 1, OC58, Protein FAM129B
计算分子量 733 aa, 83 kDa
观测分子量 83-93 kDa
GenBank蛋白编号BC067366
基因名称 FAM129B
Gene ID (NCBI) 64855
RRIDAB_2879121
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96TA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM129B, also named as Niban-like protein 1 or C9orf88, is a 746 amino acid protein, which contains one PH domain and belongs to the Niban family. FAM129B localizes in the cytoplasm and may play a role in apoptosis suppression and promote melanoma cell invasion in vitro.

实验方案

Product Specific Protocols
WB protocol for FAM129B antibody 22553-1-APDownload protocol
IHC protocol for FAM129B antibody 22553-1-APDownload protocol
FC protocol for FAM129B antibody 22553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

Characterization of PANoptosis-related genes with immunoregulatory features in osteoarthritis

Authors - Zhibin Lan
View Article