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验证数据展示

  • WB analysis of NIH/3T3 using 22553-1-AP

    WB analysis of NIH/3T3 using 22553-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22553-1-AP (FAM129B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM129B Polyclonal antibody

FAM129B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22553-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C9orf88, FAM129B, MEG 3, Niban like protein 1, OC58, Protein FAM129B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, A431 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22553-1-AP targets FAM129B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag18314

Product name:

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: of

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 129, member B
别名 C9orf88, FAM129B, MEG 3, Niban like protein 1, OC58, Protein FAM129B
计算分子量 733 aa, 83 kDa
观测分子量 83-93 kDa
GenBank蛋白编号BC067366
基因名称 FAM129B
Gene ID (NCBI) 64855
RRIDAB_2879121
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ96TA1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM129B, also named as Niban-like protein 1 or C9orf88, is a 746 amino acid protein, which contains one PH domain and belongs to the Niban family. FAM129B localizes in the cytoplasm and may play a role in apoptosis suppression and promote melanoma cell invasion in vitro.

实验方案

Product Specific Protocols
WB protocol for FAM129B antibody 22553-1-APDownload protocol
IHC protocol for FAM129B antibody 22553-1-APDownload protocol
FC protocol for FAM129B antibody 22553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int Immunopharmacol

Characterization of PANoptosis-related genes with immunoregulatory features in osteoarthritis

Authors - Zhibin Lan
View Article