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验证数据展示

  • WB analysis of human brain using 10307-1-AP

    WB analysis of human brain using 10307-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10307-1-AP (FAM127A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10307-1-AP

    IHC staining of human pancreas cancer using 10307-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10307-1-AP (FAM127A antibody) at dilution of 1:100 (under 25x lens).

FAM127A Polyclonal antibody

FAM127A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10307-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

CAAX box protein 1, CXX1, FAM127A, Mar8, MAR8C, Mart8, MART8C, Protein FAM127A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10307-1-AP targets FAM127A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

CatNo: Ag0298

Product name: Recombinant human FAM127A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 59-182 aa of BC002410

Sequence:

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 127, member A
别名 CAAX box protein 1, CXX1, FAM127A, Mar8, MAR8C, Mart8, MART8C, Protein FAM127A
计算分子量 22 kDa
观测分子量 40 kDa
GenBank蛋白编号BC002410
基因名称 FAM127A
Gene ID (NCBI) 8933
RRIDAB_2101197
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDA6ZKI3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAAX box protein 1 is also name as CXX1 or FAM127A. The function of CXX1 remains largely unknown. Catalog#10307-1-AP is a rabbit polyclonal antibody raised against the full-length of human CXX1. Two FAM127A proteins are presented in UniProt: O15255 and A6ZKI3 (UniProt Number). This antibody specially recognises the O15255.

实验方案

Product Specific Protocols
WB protocol for FAM127A antibody 10307-1-APDownload protocol
IHC protocol for FAM127A antibody 10307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Sci

The PRDM14-CtBP1/2-PRC2 complex regulates transcriptional repression during the transition from primed to naïve pluripotency.

Authors - Maiko Yamamoto
View Article