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验证数据展示

  • WB analysis of Jurkat using 25816-1-AP

    WB analysis of Jurkat using 25816-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25816-1-AP (FAM125A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 25816-1-AP

    IHC staining of human pancreas using 25816-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 25816-1-AP

    IHC staining of human pancreas using 25816-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 25816-1-AP

    IF Staining of HeLa using 25816-1-AP

    Immunofluorescent analysis of HeLa cells using 25816-1-AP (FAM125A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FAM125A Polyclonal antibody

FAM125A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25816-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CFBP, ESCRT I complex subunit MVB12A, FAM125A, Protein FAM125A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25816-1-AP targets FAM125A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FAM125A fusion protein Ag22919 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 125, member A
别名 CFBP, ESCRT I complex subunit MVB12A, FAM125A, Protein FAM125A
计算分子量 273 aa, 29 kDa
观测分子量 30 kDa
GenBank蛋白编号BC007883
基因名称 FAM125A
Gene ID (NCBI) 93343
RRIDAB_2880250
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EY5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM125A, also named as MVB12A and CFBP, is a component of the ESCRT-I complex. FAM125A is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. FAM125A has three isoforms with MW 27-28 kDa and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for FAM125A antibody 25816-1-APDownload protocol
IHC protocol for FAM125A antibody 25816-1-APDownload protocol
IF protocol for FAM125A antibody 25816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols