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FAM125A Polyclonal antibody

FAM125A Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25816-1-AP

Print datasheet

Synonyms

CFBP, ESCRT I complex subunit MVB12A, FAM125A, Protein FAM125A

验证数据展示

  • WB analysis of Jurkat using 25816-1-AP

    WB analysis of Jurkat using 25816-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25816-1-AP (FAM125A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 25816-1-AP

    IHC staining of human pancreas using 25816-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 25816-1-AP

    IHC staining of human pancreas using 25816-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25816-1-AP (FAM125A Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 25816-1-AP

    IF Staining of HeLa using 25816-1-AP

    Immunofluorescent analysis of HeLa cells using 25816-1-AP (FAM125A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25816-1-AP is FAM125A Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen FAM125A fusion protein Ag22919 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 125, member A
Synonyms CFBP, ESCRT I complex subunit MVB12A, FAM125A, Protein FAM125A
Calculated Molecular Weight 273 aa, 29 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC007883
Gene Symbol FAM125A
Gene ID (NCBI) 93343
RRIDAB_2880250
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM125A, also named as MVB12A and CFBP, is a component of the ESCRT-I complex. FAM125A is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. FAM125A has three isoforms with MW 27-28 kDa and 25 kDa.

实验方案

Product Specific Protocols
WB protocol for FAM125A antibody 25816-1-APDownload protocol
IHC protocol for FAM125A antibody 25816-1-APDownload protocol
IF protocol for FAM125A antibody 25816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols