验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21313-1-AP targets FAM124B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag15963 Product name: Recombinant human FAM124B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC025754 Sequence: MDETQGPLAMTVHLLANSGHGSLLQRTLDQLLDCICPEVRLFQVSERASPVKYCEKSHSKRSRFPGMSVLLFLHESPGEDRLFRVLDSLQHSPWQCYPTQDTRGRLCPYFFANQEFYSLDSQLPIWGVRQVHCGSEILRVTLYCSFDNYEDAIRLYEMILQREATLQKSNFCFFVLYASKSFALQLSLKQLPPGMSVDPKESSVLQFKVQEIGQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 124B |
| 别名 | FAM124B, Protein FAM124B |
| 计算分子量 | 455 aa, 51 kDa |
| 观测分子量 | 51 kDa |
| GenBank蛋白编号 | BC025754 |
| 基因名称 | FAM124B |
| Gene ID (NCBI) | 79843 |
| RRID | AB_10859248 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H5Z6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FAM124B is identified as a novel component of CHD7 and CHD8 containing complex. It is a mainly nuclear localized protein with a widespread expressed. Fam124B is a novel protein possibly involved in the pathogenesis of CHARGE syndrome and neurodevelopmental disorders.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM124B antibody 21313-1-AP | Download protocol |
| IHC protocol for FAM124B antibody 21313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Identification and characterization of FAM124B as a novel component of a CHD7 and CHD8 containing complex. | ||
Oncol Lett New prognostic factors and scoring system for patients with acute myeloid leukemia. | ||
Sci Rep Genome-wide association study of subfoveal choroidal thickness in a longitudinal cohort of older adults |
