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验证数据展示

  • WB analysis of siRNA cells using 21529-1-AP

    WB analysis of siRNA cells using 21529-1-AP

    WB result of FAM120A antibody (21529-1-AP) with siRNA control and siRNA-FAM120A.

  • WB analysis of HEK-293 using 21529-1-AP

    WB analysis of HEK-293 using 21529-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 21529-1-AP

    IHC staining of human skeletal muscle using 21529-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 21529-1-AP

    IHC staining of human skeletal muscle using 21529-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21529-1-AP

    IF Staining of HepG2 using 21529-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM120A antibody (21529-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Featured Product
  • KD/KO验证

FAM120A Polyclonal antibody

FAM120A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21529-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

C9orf10, Constitutive coactivator of PPAR-gamma-like protein 1, KIAA0183, OSSA, Oxidative stress-associated SRC activator

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, siRNA cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21529-1-AP targets FAM120A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原

CatNo: Ag16149

Product name: Recombinant human FAM120A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 285-538 aa of BC111736

Sequence: AVADYVRNIQDTSDLDAIAKDVFQHSQSRTDDKVIRFKRAIGYYSATSKPMSFHPPHYLAARPGPFGMPGMVPPHVPPQMLNIPQTSLQAKPVAPQVPSPGGAPGQGPYPYSLSEPAPLTLDTSGKNLTEQNSYSNIPHEGKHTPLYERSSPINPAQSGSPNHVDSAYFPGSSTSSSSDNDEGSGGATNHISGNKIGWEKTGSHSEPQARGDPGDQTKAEGSSTASSGSQLAEGKGSQMGTVQPIPCLLSMPTR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 120A
别名 C9orf10, Constitutive coactivator of PPAR-gamma-like protein 1, KIAA0183, OSSA, Oxidative stress-associated SRC activator
计算分子量 1118 aa, 122 kDa
观测分子量 125 kDa
GenBank蛋白编号BC111736
基因名称 FAM120A
Gene ID (NCBI) 23196
RRIDAB_10860261
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZB2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM120A antibody 21529-1-APDownload protocol
IHC protocol for FAM120A antibody 21529-1-APDownload protocol
IF protocol for FAM120A antibody 21529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Mol Neurobiol

Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay

Authors - Yifen Shen
View Article