验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33982-1-AP targets FAM119B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag40880 Product name: Recombinant human FAM119B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-70 aa of BC099841 Sequence: MADPGPDPESESESVFPREVGLFADSYSEKSQFCFCGHVLTITQNFGSRLGVAARVWDAALSLCNYFESQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 119, member B |
| 别名 | EC:2.1.1.-, EEF1A lysine methyltransferase 3, EEF1AKMT3, eEF1A-KMT3, HCA557A |
| 计算分子量 | 226 aa, 25 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC099841 |
| 基因名称 | FAM119B |
| Gene ID (NCBI) | 25895 |
| RRID | AB_3743132 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96AZ1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EEF1AKMT3 (also known as METTL21B or FAM119B) is a gene that encodes a member of the methyltransferase-like (METTL) protein family. This enzyme functions as a specific lysine methyltransferase, with its primary and well-characterized substrate being eukaryotic translation elongation factor 1 alpha (eEF1A).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FAM119B antibody 33982-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
