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验证数据展示

  • WB analysis using 21079-1-AP

    WB analysis using 21079-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21079-1-AP (FAM119A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21079-1-AP

    IF Staining of HeLa using 21079-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM119A antibody (21079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 21079-1-AP

    IF Staining of HeLa using 21079-1-AP

    Immunofluorescent analysis of HepG2 cells, using FAM119A antibody 21079-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human liver using 21079-1-AP

    IHC staining of human liver using 21079-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21079-1-AP (FAM119A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 21079-1-AP

    IHC staining of human liver using 21079-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21079-1-AP (FAM119A Antibody) at dilution of 1:200 (under 40x lens).

FAM119A Polyclonal antibody

FAM119A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21079-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

FAM119A, HCA557b, METTL21A, Protein FAM119A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, L02 cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21079-1-AP targets FAM119A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 FAM119A fusion protein Ag15282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 119, member A
别名 FAM119A, HCA557b, METTL21A, Protein FAM119A
计算分子量 218 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC033720
基因名称 FAM119A
Gene ID (NCBI) 151194
RRIDAB_10755289
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXB1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAM119A antibody 21079-1-APDownload protocol
IHC protocol for FAM119A antibody 21079-1-APDownload protocol
IF protocol for FAM119A antibody 21079-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Biol Chem

Lysine trimethylation regulates 78 kDa glucose-regulated protein proteostasis during endoplasmic reticulum stress.

Authors - Jonas Sieber
View Article
humanWB

J Proteomics

Comparative proteomic analysis of protein methylation provides insight into the resistance of hepatocellular carcinoma to 5-fluorouracil.

Authors - Zhen Liu
View Article