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验证数据展示

  • WB analysis using 67926-1-Ig (same clone as 67926-1-PBS)

    WB analysis using 67926-1-Ig (same clone as 67926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67926-1-Ig (FAM114A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67926-1-Ig (same clone as 67926-1-PBS)

    IHC staining of mouse brain using 67926-1-Ig (same clone as 67926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67926-1-Ig (FAM114A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67926-1-Ig (same clone as 67926-1-PBS)

    IF Staining of HepG2 using 67926-1-Ig (same clone as 67926-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (67926-1-Ig, Clone: 1B11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67926-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51192-1

    Cytometric bead array standard curve of MP51192-1

    Cytometric bead array standard curve of MP51192-1, FAM114A1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67926-1-PBS. Detection antibody: 67926-2-PBS. Standard:Ag17289. Range: 0.195-50 ng/mL.

FAM114A1 Monoclonal antibody, PBS Only (Capture)

FAM114A1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67926-1-PBS

产品说明书

CloneNo. 1B11D6

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Protein NOXP20, Protein FAM114A1, NOXP20, Nervous system overexpressed protein 20, 1B11D6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67926-1-PBS targets FAM114A1 as part of a matched antibody pair:

MP51192-1: 67926-1-PBS capture and 67926-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17289

Product name: Recombinant human FAM114A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 305-538 aa of BC040452

Sequence: LEILSNESESKVQSFLASLDGEKLELLKNDLISIKDIFAAKELENEENQEEQGLEEKGEEFARMLTELLFELHVAATPDKLNKAMKRAHDWVEEDQTVVSVDVAKVSEEETKKEEKEEKSQDPQEDKKEEKKTKTIEEVYMSSIESLAEVTARCIEQLHKVAELILHGQEEEKPAQDQAKVLIKLTTAMCNEVASLSKKFTNSLTTVGSNKKAEVLNPMISSVLLEGCNSTTYI

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 114, member A1
别名 Protein NOXP20, Protein FAM114A1, NOXP20, Nervous system overexpressed protein 20, 1B11D6
计算分子量 563 aa, 61 kDa
观测分子量80 kDa
GenBank蛋白编号BC040452
基因名称 FAM114A1
Gene ID (NCBI) 92689
RRIDAB_2918678
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWE2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.

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