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验证数据展示

  • WB analysis of Neuro-2a using 21638-1-AP

    WB analysis of Neuro-2a using 21638-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21638-1-AP (FAM114A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21638-1-AP

    IHC staining of mouse brain using 21638-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21638-1-AP (FAM114A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21638-1-AP

    IF Staining of HepG2 using 21638-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM114A1 antibody (21638-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

FAM114A1 Polyclonal antibody

FAM114A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21638-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

FAM114A1, Noxp20, Protein FAM114A1, Protein NOXP20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inNeuro-2a cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21638-1-AP targets FAM114A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16287

Product name: Recombinant human FAM114A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 305-538 aa of BC040452

Sequence: LEILSNESESKVQSFLASLDGEKLELLKNDLISIKDIFAAKELENEENQEEQGLEEKGEEFARMLTELLFELHVAATPDKLNKAMKRAHDWVEEDQTVVSVDVAKVSEEETKKEEKEEKSQDPQEDKKEEKKTKTIEEVYMSSIESLAEVTARCIEQLHKVAELILHGQEEEKPAQDQAKVLIKLTTAMCNEVASLSKKFTNSLTTVGSNKKAEVLNPMISSVLLEGCNSTTYI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 114, member A1
别名 FAM114A1, Noxp20, Protein FAM114A1, Protein NOXP20
计算分子量 563 aa, 61 kDa
观测分子量 80 kDa
GenBank蛋白编号BC040452
基因名称 FAM114A1
Gene ID (NCBI) 92689
RRIDAB_2878895
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWE2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM114A1, also named as Nervous system overexpressed protein 20, is a 563 amino acid protein, which belongs to the FAM114 family. FAM114A1 localizes in the cytoplasm and may play a role in neuronal cell development. The calculated molecular weight of FAM114A1 is 61 kDa, but the modified FAM114A1 may be 70-80 kDa protein.

实验方案

Product Specific Protocols
WB protocol for FAM114A1 antibody 21638-1-APDownload protocol
IHC protocol for FAM114A1 antibody 21638-1-APDownload protocol
IF protocol for FAM114A1 antibody 21638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols