验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
15854-1-AP targets ABHD17A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | ABHD17A fusion protein Ag8697 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | family with sequence similarity 108, member A1 |
别名 | FAM108A1, EC:3.1.2.22, C19orf27, Alpha/beta hydrolase domain-containing protein 17A, Abhydrolase domain-containing protein 17A |
计算分子量 | 34 kDa |
观测分子量 | 34 kDa |
GenBank蛋白编号 | BC000158 |
基因名称 | ABHD17A |
Gene ID (NCBI) | 81926 |
RRID | AB_2878193 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96GS6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha/beta hydrolase domain-containing protein 17A (ABHD17A) is a membrane-localized thioesterase, which regulates H- and N-RAS39, also catalyzes the depalmitoylation of CNAβ1 causing it to redistribute from the PM to the cytosol and the Golgi (PMID: 34663815). ABHD17a is a major acyl protein thioesterase that site-specifically deacylates the STREX domain of the BK channel to control channel activity (PMID: 32913120). Western blot analysis detected ABHD17A at an apparent molecular mass of 34 kDa (PMID: 27307232), and the 70 kDa-band could be the dimer.
实验方案
Product Specific Protocols | |
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WB protocol for ABHD17A antibody 15854-1-AP | Download protocol |
IHC protocol for ABHD17A antibody 15854-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cell Sci Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus | ||
J Biol Chem The Tumor Suppressor NF2 Modulates TEAD4 Stability and Activity in Hippo Signaling via Direct Interaction | ||
Sci Adv Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis |