ABHD17A Polyclonal antibody

ABHD17A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15854-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

FAM108A1, EC:3.1.2.22, C19orf27, Alpha/beta hydrolase domain-containing protein 17A, Abhydrolase domain-containing protein 17A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15854-1-AP targets ABHD17A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 ABHD17A fusion protein Ag8697 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 108, member A1
别名 FAM108A1, EC:3.1.2.22, C19orf27, Alpha/beta hydrolase domain-containing protein 17A, Abhydrolase domain-containing protein 17A
计算分子量 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC000158
基因名称 ABHD17A
Gene ID (NCBI) 81926
RRIDAB_2878193
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GS6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha/beta hydrolase domain-containing protein 17A (ABHD17A) is a membrane-localized thioesterase, which regulates H- and N-RAS39, also catalyzes the depalmitoylation of CNAβ1 causing it to redistribute from the PM to the cytosol and the Golgi (PMID: 34663815). ABHD17a is a major acyl protein thioesterase that site-specifically deacylates the STREX domain of the BK channel to control channel activity (PMID: 32913120). Western blot analysis detected ABHD17A at an apparent molecular mass of 34 kDa (PMID: 27307232), and the 70 kDa-band could be the dimer.

实验方案

Product Specific Protocols
WB protocol for ABHD17A antibody 15854-1-APDownload protocol
IHC protocol for ABHD17A antibody 15854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Neurosci

Identification of PSD-95 Depalmitoylating Enzymes.

Authors - Norihiko Yokoi
WB

J Cell Sci

Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus

Authors - Danya Abazari

J Biol Chem

The Tumor Suppressor NF2 Modulates TEAD4 Stability and Activity in Hippo Signaling via Direct Interaction

Authors - Mengying Wu
mouseWB

Sci Adv

Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis

Authors - Shuwei Hu
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