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FAM103A1 Polyclonal antibody

FAM103A1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19422-1-AP

Print datasheet

Synonyms

C15orf18, FAM103A1, HsT19360, Protein FAM103A1

验证数据展示

  • WB analysis of A549 using 19422-1-AP

    WB analysis of A549 using 19422-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 19422-1-AP (FAM103A1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 19422-1-AP

    IHC staining of mouse lung using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included
  • IF Staining of MCF-7 using 19422-1-AP

    IF Staining of MCF-7 using 19422-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 19422-1-AP (FAM103A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse lung using 19422-1-AP

    IHC staining of mouse lung using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included
  • IHC staining of human oesophagus cancer using 19422-1-AP

    IHC staining of human oesophagus cancer using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included
  • IHC staining of human oesophagus cancer using 19422-1-AP

    IHC staining of human oesophagus cancer using 19422-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19422-1-AP (FAM103A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RAMAC antibody included

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inmouse lung tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy RAMAC Ready-To-Use IHC Kit. RAMAC primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19422-1-AP targets FAM103A1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FAM103A1 fusion protein Ag13749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 103, member A1
Synonyms C15orf18, FAM103A1, HsT19360, Protein FAM103A1
Calculated Molecular Weight 118 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC003627
Gene Symbol FAM103A1
Gene ID (NCBI) 83640
RRIDAB_2878578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM103A1, also named as RNMT-activating mini protein, is a 118 amino acid protein, which belongs to the RAM family. FAM103A1 as a component of mRNA cap binding complex localizes in the nucleus. FAM103A1 is required for efficient mRNA cap methylation and regulates RNMT expression by a post-transcriptional stabilizing mechanism.

实验方案

Product Specific Protocols
WB protocol for FAM103A1 antibody 19422-1-APDownload protocol
IHC protocol for FAM103A1 antibody 19422-1-APDownload protocol
IF protocol for FAM103A1 antibody 19422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Mol Biol

Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.

Authors - Michael J Osborne
View Article