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验证数据展示

  • WB analysis using 81334-5-RR (same clone as 81334-5-PBS)

    WB analysis using 81334-5-RR (same clone as 81334-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81334-5-RR (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 81334-5-RR (same clone as 81334-5-PBS)

    IF Staining of A549 using 81334-5-RR (same clone as 81334-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 81334-5-RR (same clone as 81334-5-PBS)

    IF Staining of MCF-7 using 81334-5-RR (same clone as 81334-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81334-5-RR

    Affinity and Kinetic Characterization of 81334-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81334-5-RR against Human FAK were performed. The affinity constant is 2.03 nM.

FAK Recombinant monoclonal antibody, PBS Only

FAK Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81334-5-PBS

产品说明书

CloneNo. 242295E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.

产品信息

81334-5-PBS targets FAK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3331

Product name: Recombinant human FAK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 381-678 aa of BC028733

Sequence: ITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PTK2 protein tyrosine kinase 2
别名 PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1
计算分子量 1052 aa, 119 kDa
观测分子量 110 kDa
GenBank蛋白编号BC028733
基因名称 FAK
Gene ID (NCBI) 5747
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ05397
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971).

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