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FAIM Polyclonal antibody

FAIM Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 13005-1-AP

Print datasheet

Synonyms

FAIM, FAIM1

验证数据展示

  • IP experiment of HEK-293 using 13005-1-AP

    IP experiment of HEK-293 using 13005-1-AP

    IP result of anti-FAIM (IP:13005-1-AP, 4ug; Detection:13005-1-AP 1:500) with HEK-293 cells lysate 1680 ug.

  • WB analysis of HEK-293 using 13005-1-AP

    WB analysis of HEK-293 using 13005-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13005-1-AP (FAIM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 13005-1-AP

    IHC staining of human gliomas using 13005-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13005-1-AP (FAIM antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13005-1-AP targets FAIM in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen FAIM fusion protein Ag3685 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fas apoptotic inhibitory molecule
Synonyms FAIM, FAIM1
Calculated Molecular Weight 179 aa, 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC012478
Gene Symbol FAIM
Gene ID (NCBI) 55179
RRIDAB_2100935
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FAIM antibody 13005-1-APDownload protocol
IHC protocol for FAIM antibody 13005-1-APDownload protocol
IP protocol for FAIM antibody 13005-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

Autophagy

FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma.

Authors - Tianyu Han
View Article