• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 15205-1-AP

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 15205-1-AP

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 15205-1-AP

    IP experiment of L02 using 15205-1-AP

    IP result of anti-FADS3 (IP:15205-1-AP, 4ug; Detection:15205-1-AP 1:500) with L02 cells lysate 3000 ug.

  • WB analysis using 15205-1-AP

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 15205-1-AP

    IHC staining of human ovary cancer using 15205-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15205-1-AP (FADS3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FADS3 Polyclonal antibody

FADS3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15205-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

fatty acid desaturase 3, FADS 3, EC:1.14.19.-, Delta(13) fatty acid desaturase (Delta(13) desaturase ), Cytochrome b5 related protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, mouse kidney tissue, L02 cells, rat skeletal muscle tissue, mouse liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15205-1-AP targets FADS3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FADS3 fusion protein Ag7355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid desaturase 3
别名 fatty acid desaturase 3, FADS 3, EC:1.14.19.-, Delta(13) fatty acid desaturase (Delta(13) desaturase ), Cytochrome b5 related protein
计算分子量 51 kDa
观测分子量 41-51 kDa
GenBank蛋白编号BC004901
基因名称 FADS3
Gene ID (NCBI) 3995
RRIDAB_10859776
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5Q0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FADS3, also named as CYB5RP, belongs to fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family memebers are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase protion, both of which are characterized by conserved histidine motifs. FADS3 can be detected as 40 kDa-51 kDa.

实验方案

Product Specific Protocols
WB protocol for FADS3 antibody 15205-1-APDownload protocol
IHC protocol for FADS3 antibody 15205-1-APDownload protocol
IP protocol for FADS3 antibody 15205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols