• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FADS3 Polyclonal antibody

FADS3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15205-1-AP

Print datasheet

Synonyms

CYB5RP, Cytochrome b5 related protein, FADS3, fatty acid desaturase 3, LLCDL3

验证数据展示

  • WB analysis using 15205-1-AP

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis using 15205-1-AP

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 15205-1-AP

    IP experiment of L02 using 15205-1-AP

    IP result of anti-FADS3 (IP:15205-1-AP, 4ug; Detection:15205-1-AP 1:500) with L02 cells lysate 3000 ug.

  • WB analysis using 15205-1-AP

    WB analysis using 15205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, mouse kidney tissue, L02 cells, rat skeletal muscle tissue, mouse liver tissue
Positive IP detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15205-1-AP targets FADS3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen FADS3 fusion protein Ag7355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fatty acid desaturase 3
Synonyms CYB5RP, Cytochrome b5 related protein, FADS3, fatty acid desaturase 3, LLCDL3
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 41-51 kDa
GenBank Accession NumberBC004901
Gene Symbol FADS3
Gene ID (NCBI) 3995
RRIDAB_10859776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Q0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FADS3, also named as CYB5RP, belongs to fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family memebers are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase protion, both of which are characterized by conserved histidine motifs. FADS3 can be detected as 40 kDa-51 kDa.

实验方案

Product Specific Protocols
WB protocol for FADS3 antibody 15205-1-APDownload protocol
IP protocol for FADS3 antibody 15205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols