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验证数据展示

  • WB analysis of L02 using 84527-4-RR (same clone as 84527-4-PBS)

    WB analysis of L02 using 84527-4-RR (same clone as 84527-4-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84527-4-PBS in a different storage buffer formulation.

  • WB analysis using 84527-4-RR (same clone as 84527-4-PBS)

    WB analysis using 84527-4-RR (same clone as 84527-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84527-4-PBS in a different storage buffer formulation.

  • WB analysis using 84527-4-RR (same clone as 84527-4-PBS)

    WB analysis using 84527-4-RR (same clone as 84527-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84527-4-PBS in a different storage buffer formulation.

  • IF Staining of L02 using 84527-4-RR (same clone as 84527-4-PBS)

    IF Staining of L02 using 84527-4-RR (same clone as 84527-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using FADS2 antibody (84527-4-RR, Clone: 241863D2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84527-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84527-4-RR

    Affinity and Kinetic Characterization of 84527-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84527-4-RR against Human FADS2-Specific were performed. The affinity constant is below 1 pM.

FADS2 Recombinant monoclonal antibody, PBS Only

FADS2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84527-4-PBS

产品说明书

CloneNo. 241863D2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

241863D2, Acyl-CoA 6-desaturase, D6D, Delta-6 desaturase, EC:1.14.19.3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84527-4-PBS targets FADS2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27715

Product name: Recombinant human FADS2-Specific protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-124 aa of BC009011

Sequence: MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 fatty acid desaturase 2
别名 241863D2, Acyl-CoA 6-desaturase, D6D, Delta-6 desaturase, EC:1.14.19.3
计算分子量 445 aa, 49 kDa
观测分子量45 kDa
GenBank蛋白编号BC009011
基因名称 FADS2
Gene ID (NCBI) 9415
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO95864
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.

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