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FADS2 Polyclonal antibody

FADS2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28034-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (2)

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inL02 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28034-1-AP targets FADS2 in WB, IHC, IF, ELISA, ICC-IF applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, ICC-IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 FADS2 fusion protein Ag27709 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid desaturase 2
别名 EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase
计算分子量 445 aa, 49 kDa
观测分子量 46-52 kDa
GenBank蛋白编号BC009011
基因名称 FADS2
Gene ID (NCBI) 9415
RRIDAB_2918142
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95864
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.

实验方案

Product Specific Protocols
WB protocol for FADS2 antibody 28034-1-APDownload protocol
IHC protocol for FADS2 antibody 28034-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Chem Biol

FADS2-dependent fatty acid desaturation dictates cellular sensitivity to ferroptosis and permissiveness for hepatitis C virus replication.

Authors - Daisuke Yamane
  • KD Validated
humanWB

FASEB J

PMID: 31199678

Authors - Ge Liu
human,mouseWB,IF

Immun Inflamm Dis

Identification of Ferroptosis-Related Gene in Age-Related Macular Degeneration Using Machine Learning

Authors - Meijiang Zhu
ICC-IF

BME Front

Multimodal Metabolic Imaging Reveals Pigment Reduction and Lipid Accumulation in Metastatic Melanoma

Authors - Hyeon Jeong Lee
mouseWB

Int J Mol Sci

The Effects of Early-Life Stress on Liver Transcriptomics and the Protective Role of EPA in a Mouse Model of Early-Life-Stress-Induced Adolescent Depression

Authors - Jinlan Zhao
human,mouseWB

Int Immunopharmacol

E3 ubiquitin ligase Siah1 aggravates NAFLD through Scp2 ubiquitination

Authors - Zhu Zhu
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