FADS2 Monoclonal antibody
FADS2 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat and More (2)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G4B10
验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, A549 cells, LNCaP cells, U2OS cells, Jurkat cells, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
68026-1-Ig targets FADS2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Immunogen | FADS2 fusion protein Ag27715 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | fatty acid desaturase 2 |
| Synonyms | D6D, Delta(6) desaturase, Delta(6) fatty acid desaturase, DES6, FADS2, FADSD6, fatty acid desaturase 2, LLCDL2, SLL0262, TU13 |
| Calculated Molecular Weight | 445 aa, 49 kDa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | BC009011 |
| Gene Symbol | FADS2 |
| Gene ID (NCBI) | 9415 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95864 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FADS2 antibody 68026-1-Ig | Download protocol |
| IHC protocol for FADS2 antibody 68026-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proteomics Proteomics coupled transcriptomics reveals lipopolysaccharide inhibiting peroxisome proliferator-activated receptors signalling pathway to reduce lipid droplets accumulation in mouse liver | ||
Front Genet Identifification and validation of ferroptosis signatures and immune infifiltration characteristics associated with intervertebral disc degeneration |
