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验证数据展示

  • WB analysis of A375 using 66299-1-Ig (same clone as 66299-1-PBS)

    WB analysis of A375 using 66299-1-Ig (same clone as 66299-1-PBS)

    WB result of FABP5 antibody (66299-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • WB analysis using 66299-1-Ig (same clone as 66299-1-PBS)

    WB analysis using 66299-1-Ig (same clone as 66299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • WB analysis of Hepa1-6 using 66299-1-Ig (same clone as 66299-1-PBS)

    WB analysis of Hepa1-6 using 66299-1-Ig (same clone as 66299-1-PBS)

    WB result of FABP5 antibody (66299-1-Ig; 1:1000; room temperature for 1.5 hours) with wild-type and FABP5 knockout Hepa1-6 cells. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brown adipose using 66299-1-Ig (same clone as 66299-1-PBS)

    IHC staining of mouse brown adipose using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP5 antibody included
  • IF Staining of HepG2 using 66299-1-Ig (same clone as 66299-1-PBS)

    IF Staining of HepG2 using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FABP5 antibody (66299-1-Ig, Clone: 1C6E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brown adipose using 66299-1-Ig (same clone as 66299-1-PBS)

    IHC staining of mouse brown adipose using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP5 antibody included
  • WB analysis of A375 using 66299-1-Ig (same clone as 66299-1-PBS)

    WB analysis of A375 using 66299-1-Ig (same clone as 66299-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    IHC staining of human breast cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP5 antibody included
  • IHC staining of human breast cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    IHC staining of human breast cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP5 antibody included
  • WB analysis using 66299-1-Ig (same clone as 66299-1-PBS)

    WB analysis using 66299-1-Ig (same clone as 66299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    IHC staining of human prostate cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP5 antibody included
  • IHC staining of human prostate cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    IHC staining of human prostate cancer using 66299-1-Ig (same clone as 66299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FABP5 antibody included
  • Featured Product
  • KD/KO验证

FABP5 Monoclonal antibody, PBS Only

FABP5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66299-1-PBS

产品说明书

CloneNo. 1C6E12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

1C6E12, EFABP, Epidermal-type fatty acid-binding protein, Fatty acid binding protein 5, Fatty acid-binding protein 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FABP5 Ready-To-Use IHC Kit. FABP5 primary antibody included.

产品信息

66299-1-PBS targets FABP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3005

Product name: Recombinant human FABP5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-135 aa of BC019385

Sequence: MATVQQLEGRWRLVDSKGFDEYMKELGVGIALRKMGAMAKPDCIITCDGKNLTIKTESTLKTTQFSCTLGEKFEETTADGRKTQTVCNFTDGALVQHQEWDGKESTITRKLKDGKLVVECVMNNVTCTRIYEKVE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 fatty acid binding protein 5 (psoriasis-associated)
别名 1C6E12, EFABP, Epidermal-type fatty acid-binding protein, Fatty acid binding protein 5, Fatty acid-binding protein 5
计算分子量 135 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC019385
基因名称 FABP5
Gene ID (NCBI) 2171
RRIDAB_2881682
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ01469
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

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