验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, MC38 cells, RT-4 cells, mouse adipose tissue, mouse skeletal muscle tissue, rat heart tissue, human adipose tissue, rat adipose tissue |
| Positive IHC detected in | mouse skin tissue, human heart tissue, human ovary tumor tissue, rat brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12802-1-AP targets FABP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, bovine, hamster, sheep, goat, geese |
| 免疫原 |
CatNo: Ag3912 Product name: Recombinant mouse Fabp4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-132 aa of BC054426 Sequence: MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDLVTIRSESTFKNTEISFKLGVEFDEITADDRKVKSIITLDGGALVQVQKWDGKSTTIKRKRDGDKLVVECVMKGVTSTRVYERA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fatty acid binding protein 4, adipocyte |
| 别名 | Adipocyte lipid-binding protein, Adipocyte-type fatty acid-binding protein, AFABP, ALBP, Fatty acid binding protein 4 |
| 计算分子量 | 132 aa, 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC003672 |
| 基因名称 | FABP4 |
| Gene ID (NCBI) | 2167 |
| RRID | AB_2102442 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15090 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for FABP4 antibody 12802-1-AP | Download protocol |
| IHC protocol for FABP4 antibody 12802-1-AP | Download protocol |
| WB protocol for FABP4 antibody 12802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Targeting OxLDL-mediated CD36 + CAF reprogramming potentiates PD-1 immunotherapy in osteosarcoma | ||
Cell Metab Adipokine IL-11/IL-11Ra constrains sphingolipid metabolism to limit the thermogenic capacity of beige adipocytes. | ||
Bone Res NELL2, a novel osteoinductive factor, regulates osteoblast differentiation and bone homeostasis through fibronectin 1/integrin-mediated FAK/AKT signaling | ||
Autophagy m6A mRNA methylation controls autophagy and adipogenesis by targeting Atg5 and Atg7 | ||
Nat Commun Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate. |
