验证数据展示
经过测试的应用
Positive WB detected in | MKN-45 cells, SGC-7901 cells, fetal human brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86020-2-RR targets FA2H in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | FA2H fusion protein Ag7717 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | fatty acid 2-hydroxylase |
别名 | EC:1.14.18.-, FAAH, Fatty acid 2-hydroxylase, Fatty acid alpha-hydroxylase, Fatty acid hydroxylase domain-containing protein 1 |
计算分子量 | 43 kDa |
观测分子量 | 43 kDa |
GenBank蛋白编号 | BC002679 |
基因名称 | FA2H |
Gene ID (NCBI) | 79152 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q7L5A8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FA2H(Fatty acid 2-hydroxylase) required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids. The deduced 372-amino acid protein has a calculated molecular mass of 42.8 kD .Both mouse and human FA2H have a C-terminal endoplasmic reticulum (ER) retention motif(PMID: 15658937).
实验方案
Product Specific Protocols | |
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WB protocol for FA2H antibody 86020-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |