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验证数据展示

  • WB analysis using 32696-1-AP (same clone as 32696-1-PBS)

    WB analysis using 32696-1-AP (same clone as 32696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32696-1-AP (F8A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32696-1-PBS in a different storage buffer formulation.

  • WB analysis using 32696-1-AP (same clone as 32696-1-PBS)

    WB analysis using 32696-1-AP (same clone as 32696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32696-1-AP (F8A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32696-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 32696-1-AP (same clone as 32696-1-PBS)

    IF Staining of HeLa using 32696-1-AP (same clone as 32696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using F8A2 antibody (32696-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32696-1-PBS in a different storage buffer formulation.

F8A2 Polyclonal antibody, PBS Only

F8A2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 32696-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

40-kDa huntingtin-associated protein, Coagulation Factor VIII Associated 2, CpG island protein, F8A1, F8A3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32696-1-PBS targets F8A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag38529

Product name: Recombinant human F8A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 20-216 aa of NM_012151.3

Sequence: GDFLARYRLVSNKLKKRFLRKPNVAEAGEQFGQLGRELRAQECLPYAAWCQLAVARCQQALFHGPGEALALTEAARLFLRQERDARQRLVCPAAYGEPLQAAASALGAAVRLHLELGQPAAAAALCLELAAALRDLGQPAAAAGHFQRAAQLQLPQLPLAALQALGEAASCQLLARDYTGALAVFTRMQRLAREHGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coagulation factor VIII-associated (intronic transcript) 2
别名 40-kDa huntingtin-associated protein, Coagulation Factor VIII Associated 2, CpG island protein, F8A1, F8A3
计算分子量39kDa，371aa
观测分子量38 kDa
GenBank蛋白编号NM_012151.3
基因名称 F8A2
Gene ID (NCBI) 474383
RRIDAB_3742713
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP23610
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

F8A2 (Coagulation Factor VIII Associated 2) is a protein associated with coagulation factor VIII, which is widely expressed in a variety of cell types, localized mainly in the cytoplasm and nucleus, and also co-localized with Huntington's protein (HTT). Although its specific function is not fully defined, F8A2 may play an important role in intracellular and vesicular transport. In addition, F8A2 co-localizes with ubiquitin in neuronal cells and may be involved in the pathological process of neurodegenerative diseases.

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