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F7 Polyclonal antibody

F7 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23058-1-AP

Print datasheet

Synonyms

Coagulation factor VII, Eptacog alfa, F7, Proconvertin, SPCA

验证数据展示

  • WB analysis of human placenta using 23058-1-AP

    WB analysis of human placenta using 23058-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HUVEC using 23058-1-AP

    IF Staining of HUVEC using 23058-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 23058-1-AP (F7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, human plasma tissue, mouse liver tissue
Positive IF detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 23058-1-AP is F7 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen F7 fusion protein Ag19324 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name coagulation factor VII (serum prothrombin conversion accelerator)
Synonyms Coagulation factor VII, Eptacog alfa, F7, Proconvertin, SPCA
Calculated Molecular Weight 466 aa, 52 kDa
Observed Molecular Weight 49-55 kDa
GenBank Accession NumberBC130468
Gene Symbol F7
Gene ID (NCBI) 2155
RRIDAB_2879203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP08709
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

F7(Coagulation factor VII) is also named as serum prothrombin conversion accelerator(SPCA). It is the key triggering enzyme of the extrinsic coagulation pathway. Hepatocytes are the predominant source of F7 production under normal physiologic conditions, although F7 is also expressed by monocytes/macrophages in inflammation and atherosclerosis(PMID:17018600). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for F7 antibody 23058-1-APDownload protocol
IF protocol for F7 antibody 23058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
View Article