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验证数据展示

  • WB analysis using 26366-1-AP

    WB analysis using 26366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26366-1-AP (PAR1/Thrombin Receptor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 26366-1-AP

    WB analysis of K-562 using 26366-1-AP

    WB result of F2R antibody (26366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAR1/Thrombin Receptor transfected K-562 cells.

  • IHC staining of human liver using 26366-1-AP

    IHC staining of human liver using 26366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 26366-1-AP

    IHC staining of human liver using 26366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

PAR1/Thrombin Receptor Polyclonal antibody

PAR1/Thrombin Receptor Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26366-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, ELISA and More (1)

别名

F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, K-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26366-1-AP targets PAR1/Thrombin Receptor in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human
文献引用反应性human, mouse, hamster
免疫原 PAR1/Thrombin Receptor fusion protein Ag24378 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coagulation factor II (thrombin) receptor
别名 F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1
计算分子量 47 kDa
观测分子量70 kDa
GenBank蛋白编号BC051909
基因名称 F2R
Gene ID (NCBI) 2149
RRIDAB_2880492
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25116
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)

实验方案

Product Specific Protocols
WB protocol for PAR1/Thrombin Receptor antibody 26366-1-APDownload protocol
IHC protocol for PAR1/Thrombin Receptor antibody 26366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Breast Cancer Res Treat

Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.

Authors - Yihui Zhu
View Article
hamsterWB

Leukemia

Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15

Authors - Cong Liu
View Article