验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, K-562 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26366-1-AP targets PAR1/Thrombin Receptor in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, monkey, hamster |
| 免疫原 |
CatNo: Ag24378 Product name: Recombinant human F2R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 44-102 aa of BC051909 Sequence: LLRNPNDKYEPFWEDEEKNESGLTEYRLVSINKSSPLQKQLPAFISEDASGYLTSSWLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coagulation factor II (thrombin) receptor |
| 别名 | F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1 |
| 计算分子量 | 47 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC051909 |
| 基因名称 | F2R |
| Gene ID (NCBI) | 2149 |
| RRID | AB_2880492 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P25116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PAR1/Thrombin Receptor antibody 26366-1-AP | Download protocol |
| WB protocol for PAR1/Thrombin Receptor antibody 26366-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Breast Cancer Res Treat Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT. | ||
Leukemia Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15 | ||
Genes Immun A Golgi apparatus-related signature predicts the immune microenvironment and prognosis of gastric cancer |
