• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Factor XII Monoclonal antibody

Factor XII Monoclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

2E2F11

Cat No : 66089-1-Ig

Print datasheet

Synonyms

Factor XII, Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor

验证数据展示

  • WB analysis of K-562 using 66089-1-Ig

    WB analysis of K-562 using 66089-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66089-1-Ig (Factor XII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse liver using 66089-1-Ig

    IF Staining of mouse liver using 66089-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Factor XII antibody (66089-1-Ig, Clone: 2E2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HepG2 using 66089-1-Ig

    IF Staining of HepG2 using 66089-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66089-1-Ig(Factor XII antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver using 66089-1-Ig

    IHC staining of human liver using 66089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 66089-1-Ig

    IHC staining of human liver using 66089-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66089-1-Ig (Factor XII antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inK562 cells, K-562 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse liver tissue, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 66089-1-Ig is Factor XII Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Factor XII fusion protein Ag3401 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name coagulation factor XII (Hageman factor)
Synonyms Factor XII, Coagulation factor XII, F12, HAE3, HAEX, HAF, Hageman factor
Calculated Molecular Weight 615 aa, 68 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC012390
Gene Symbol F12
Gene ID (NCBI) 2161
RRIDAB_11232031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

实验方案

Product Specific Protocols
WB protocol for Factor XII antibody 66089-1-IgDownload protocol
IHC protocol for Factor XII antibody 66089-1-IgDownload protocol
IF protocol for Factor XII antibody 66089-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Ther Nucleic Acids

Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.

Authors - Fanfan Li
View Article