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F11R Polyclonal antibody

F11R Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26941-1-AP

Print datasheet

Synonyms

CD321, F11 receptor, F11R, JAM, JAM 1, JAM A, JAM1, JAMA, JAMA1, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, PAM 1, Platelet adhesion molecule 1, Platelet F11 receptor

验证数据展示

  • IHC staining of human breast cancer using 26941-1-AP

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26941-1-AP

    IHC staining of human breast cancer using 26941-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U-87 MG using 26941-1-AP

    FC experiment of U-87 MG using 26941-1-AP

    1X10^6 U-87 MG cells were surface stained with 0.8 ug Anti-Human F11R (26941-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were not fixed .

  • WB analysis using 26941-1-AP

    WB analysis using 26941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHUVEC cells, human peripheral blood platelets, U-87 MG cells
Positive IHC detected inhuman breast cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inU-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26941-1-AP targets F11R in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity human
Immunogen F11R fusion protein Ag25641 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name F11 receptor
Synonyms CD321, F11 receptor, F11R, JAM, JAM 1, JAM A, JAM1, JAMA, JAMA1, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, PAM 1, Platelet adhesion molecule 1, Platelet F11 receptor
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001533
Gene Symbol F11R
Gene ID (NCBI) 50848
RRIDAB_2880692
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y624
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and ligand for the integrin LFA1.

实验方案

Product Specific Protocols
WB protocol for F11R antibody 26941-1-APDownload protocol
IHC protocol for F11R antibody 26941-1-APDownload protocol
FC protocol for F11R antibody 26941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols