验证数据展示
经过测试的应用
| Positive WB detected in | Caki-1 cells, SKOV-3 cells, U-87 MG cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55080-1-AP targets Ephrin B3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ephrin-B3 |
| 别名 | EFNB3, EFL6, EPLG8, LERK 8, LERK8 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | NM_001406 |
| 基因名称 | EFNB3 |
| Gene ID (NCBI) | 1949 |
| RRID | AB_3743199 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15768 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ephrin-B3 is a transmembrane ligand that binds primarily to EphB receptors to mediate bidirectional cell signaling, crucial for neural development, axon guidance, and synaptic plasticity. Highly expressed in the brain and spinal cord, it acts as a midline repellent to guide axonal pathfinding (PMID: 16951161). Ephrin‑B3 is important in the regulation of cell proliferation, differentiation and migration via cell‑cell contact, and can activate the reelin pathway during brain development. The band of ephrin-B3 was identical to phosphorylated ephrin-B at 50 kDa (PMID: 16951161, 21447033).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Ephrin B3 antibody 55080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
