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验证数据展示

  • WB analysis using 26533-1-AP (same clone as 26533-1-PBS)

    WB analysis using 26533-1-AP (same clone as 26533-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26533-1-AP (Ephrin B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26533-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 26533-1-AP (same clone as 26533-1-PBS)

    IF Staining of SH-SY5Y using 26533-1-AP (same clone as 26533-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Ephrin B2 antibody (26533-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 26533-1-PBS in a different storage buffer formulation.

Ephrin B2 Polyclonal antibody, PBS Only

Ephrin B2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 26533-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

EFNB2, EPLG5, HTK ligand, Htk L, Ephrin-B2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26533-1-PBS targets Ephrin B2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24865

Product name: Recombinant human EFNB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 168-235 aa of BC074856

Sequence: ASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAGIASGC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ephrin-B2
别名 EFNB2, EPLG5, HTK ligand, Htk L, Ephrin-B2
计算分子量 333 aa, 37 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC074856
基因名称 EFNB2
Gene ID (NCBI) 1948
RRIDAB_3085878
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52799
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ephrin B2 (EFNB2) is a transmembrane ligand for the Ephrin B class (EPHB) of receptor tyrosine kinases. EPHB receptor(EPHB1-EPHB4, EPH6) are located at the member of one cell and activated by binding EFNB protein located on another cell, resulting in a bidirectional Ephrin-EphR signaling (PMID: 26790531). Ephrin-B are involved in vasculogenesis, neural development and synaptic plasticity, and in pathological processes such as cancer or neurodegeneration (PMID:29508392). The calculated molecular weight of EFNB2 is 37 kDa, and there is also detected about 50 kDa band in the article (PMID: 27650867, PMID: 23492730).

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