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验证数据展示

  • WB analysis of mouse kidney using 67310-1-Ig

    WB analysis of mouse kidney using 67310-1-Ig

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 67310-1-Ig

    IHC staining of human ovary tumor using 67310-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 67310-1-Ig

    IHC staining of human ovary tumor using 67310-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 67310-1-Ig

    IF Staining of human placenta using 67310-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Entactin antibody (67310-1-Ig, Clone: 2A11G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 67310-1-Ig

    IF Staining of HEK-293 using 67310-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Entactin antibody (67310-1-Ig, Clone: 2A11G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

Entactin Monoclonal antibody

Entactin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67310-1-Ig

产品说明书

CloneNo. 2A11G1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Mouse, Rat

应用

WB, IHC, ELISA

别名

Entactin, NID, NID 1, NID1, nidogen 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue, HeLa cells, human placenta tissue, pig heart tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67310-1-Ig targets Entactin in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Pig, Mouse, Rat
免疫原

CatNo: Ag4739

Product name: Recombinant human NID1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 28-400 aa of BC045606

Sequence: CLSRQELFPFGPGQGDLELEDGDDFVSPALELRGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESIENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nidogen 1
别名 Entactin, NID, NID 1, NID1, nidogen 1
计算分子量 1247 aa, 136 kDa
观测分子量 150 kDa
GenBank蛋白编号BC045606
基因名称 Entactin/NID
Gene ID (NCBI) 4811
RRIDAB_2882570
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP14543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Entactin antibody 67310-1-IgDownload protocol
WB protocol for Entactin antibody 67310-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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