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验证数据展示

  • WB analysis using 27871-1-AP (same clone as 27871-1-PBS)

    WB analysis using 27871-1-AP (same clone as 27871-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27871-1-AP (Endothelin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

    IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

    IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

    IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

    IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 27871-1-AP (same clone as 27871-1-PBS)

    IF Staining of NIH/3T3 using 27871-1-AP (same clone as 27871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

  • IF Staining of BxPC-3 using 27871-1-AP (same clone as 27871-1-PBS)

    IF Staining of BxPC-3 using 27871-1-AP (same clone as 27871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

Endothelin 3 Polyclonal antibody, PBS Only

Endothelin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27871-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EDN3, Endothelin-3, ET 3, ET3, ET-3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27871-1-PBS targets Endothelin 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag27393

Product name: Recombinant human EDN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-198 aa of BC053866

Sequence: SQSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAEKTDKEEEGKVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endothelin 3
别名 EDN3, Endothelin-3, ET 3, ET3, ET-3
观测分子量 28 kDa
GenBank蛋白编号BC053866
基因名称 Endothelin 3
Gene ID (NCBI) 1908
RRIDAB_3086000
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14138
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endothelin 3 (EDN3) is also named as ET-3 and Preproendothelin-3 (PPET3). Endothelin 3 belongs to the endothelin/sarafotoxin family. It reported downregulation of EDN3 as a result of methylation of its promoter in breast cancer, and EDN3 level was also decreased in cervical cancer (PMID: 36542159). ET-3 is the most abundant ET peptide in the rat brain, mainly localized to neurons and glia of the neostriatum, hypothalamic nuclei, hippocampus, and Purkinje cells of the cerebellum and medulla oblongata (PMID: 32589590).

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