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验证数据展示

  • WB analysis using 11348-1-AP (same clone as 11348-1-PBS)

    WB analysis using 11348-1-AP (same clone as 11348-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11348-1-AP (EXT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

    IHC staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11348-1-AP (EXT2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

    IF Staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

    IF Staining of human colon cancer using 11348-1-AP (same clone as 11348-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 11348-1-AP (same clone as 11348-1-PBS)

    IF Staining of Jurkat using 11348-1-AP (same clone as 11348-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using EXT2 antibody (11348-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11348-1-PBS in a different storage buffer formulation.

EXT2 Polyclonal antibody, PBS Only

EXT2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 11348-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

EC:2.4.1.224, Exostosin 2, EXT-2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Heparan sulfate co-polymerase subunit EXT1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11348-1-PBS targets EXT2 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1748

Product name: Recombinant human EXT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 369-718 aa of BC010058

Sequence: KMSDVYSILQSIPQRQIEEMQRQARWFWEAYFQSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWGSVSNPLFLPLIPPQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEDSLWPKIRVPLKVVRTAENKLSNRFFPYDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGYPGRLHLWDHEMNKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHMNCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exostoses (multiple) 2
别名 EC:2.4.1.224, Exostosin 2, EXT-2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Heparan sulfate co-polymerase subunit EXT1
计算分子量 718 aa, 82 kDa
观测分子量 70-82 kDa
GenBank蛋白编号BC010058
基因名称 EXT2
Gene ID (NCBI) 2132
RRIDAB_2293839
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93063
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Exostosin-2 (EXT2) is type II transmembrane glycosyltransferase forming with EXT1 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone(PMID: 22660413, 36402845, 36593275).

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