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验证数据展示

  • WB analysis using 15062-1-AP (same clone as 15062-1-PBS)

    WB analysis using 15062-1-AP (same clone as 15062-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

  • WB analysis using 15062-1-AP (same clone as 15062-1-PBS)

    WB analysis using 15062-1-AP (same clone as 15062-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15062-1-AP (EXOSC3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15062-1-AP (same clone as 15062-1-PBS)

    IHC staining of human colon cancer using 15062-1-AP (same clone as 15062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EXOSC3 antibody included
  • IHC staining of human colon cancer using 15062-1-AP (same clone as 15062-1-PBS)

    IHC staining of human colon cancer using 15062-1-AP (same clone as 15062-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15062-1-AP (EXOSC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EXOSC3 antibody included
  • IF Staining of PC-3 using 15062-1-AP (same clone as 15062-1-PBS)

    IF Staining of PC-3 using 15062-1-AP (same clone as 15062-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using EXOSC3 antibody (15062-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

  • IP experiment of A2780 using 15062-1-AP (same clone as 15062-1-PBS)

    IP experiment of A2780 using 15062-1-AP (same clone as 15062-1-PBS)

    IP result of anti-EXOSC3 (IP:15062-1-AP, 4ug; Detection:15062-1-AP 1:500) with A2780 cells lysate 800ug. This data was developed using the same antibody clone with 15062-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EXOSC3 Polyclonal antibody, PBS Only

EXOSC3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 15062-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EXOSC3 Ready-To-Use IHC Kit. EXOSC3 primary antibody included.

产品信息

15062-1-PBS targets EXOSC3 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7065

Product name: Recombinant human EXOSC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-275 aa of BC002437

Sequence: MAEPASVAAESLAGSRARAARTVLGQVVLPGEELLLPEQEDAEGPGGAVERPLSLNARACSRVRVVCGPGLRRCGDRLLVTKCGRLRHKEPGSGSGGGVYWVDSQQKRYVPVKGDHVIGIVTAKSGDIFKVDVGGSEPASLSYLSFEGATKRNRPNVQVGDLIYGQFVVANKDMEPEMVCIDSCGRANGMGVIGQDGLLFKVTLGLIRKLLAPDCEIIQEVGKLYPLEIVFGMNGRIWVKAKTIQQTLILANILEACEHMTSDQRKQIFSRLAES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exosome component 3
别名
计算分子量 30 kDa
观测分子量 31 kDa
GenBank蛋白编号BC002437
基因名称 EXOSC3
Gene ID (NCBI) 51010
RRIDAB_2278183
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQT5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA exosomes are multi-subunit complexes conserved throughout evolution, and they are emerging as the major cellular machinery for processing, surveillance and turnover of a diverse spectrum of coding and noncoding RNA substrates essential for viability[PMID:22544365]. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts [PMID:11782436]. EXOSC3 is a non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of Rnase PH-domain subunits through contacts with EXOSC9 and EXOSC5 [PMID:21255825].

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