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EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16731-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC10 Ready-To-Use IHC Kit. EXOSC10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16731-1-AP targets EXOSC10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 EXOSC10 fusion protein Ag10189 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exosome component 10
别名 Autoantigen PM/Scl 2, EXOSC10, exosome component 10, p2, p3, p4, PM Scl, PM/Scl 100, PMSCL, PMSCL2, RRP6, Rrp6p
计算分子量 885 aa, 101 kDa
观测分子量 100 kDa
GenBank蛋白编号BC073788
基因名称 EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2101730
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01780
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

实验方案

Product Specific Protocols
WB protocol for EXOSC10 antibody 16731-1-APDownload protocol
IHC protocol for EXOSC10 antibody 16731-1-APDownload protocol
IF protocol for EXOSC10 antibody 16731-1-APDownload protocol
IP protocol for EXOSC10 antibody 16731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

PeerJ

EXOSC10 is a novel hepatocellular carcinoma prognostic biomarker: a comprehensive bioinformatics analysis and experiment verification

Authors - Zhi-Yong Meng
  • KD Validated
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