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  • Featured Product
  • KD/KO Validated

EXOSC10 Polyclonal antibody

EXOSC10 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11178-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 11178-1-AP

    WB analysis of HeLa using 11178-1-AP

    WB result of EXOSC10 antibody (11178-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

  • IP experiment of MCF-7 using 11178-1-AP

    IP experiment of MCF-7 using 11178-1-AP

    IP result of anti-EXOSC10 (IP:11178-1-AP, 4ug; Detection:11178-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

  • IHC staining of human breast cancer using 11178-1-AP

    IHC staining of human breast cancer using 11178-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with EXOSC10 antibody included
  • IF Staining of NIH/3T3 using 11178-1-AP

    IF Staining of NIH/3T3 using 11178-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy EXOSC10 Ready-To-Use IHC Kit. EXOSC10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11178-1-AP targets EXOSC10 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen EXOSC10 fusion protein Ag1666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exosome component 10
Synonyms
Calculated Molecular Weight 98 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC039901
Gene Symbol EXOSC10
Gene ID (NCBI) 5394
RRIDAB_2293792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01780
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a neuclear
ucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl 2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl paitents. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

实验方案

Product Specific Protocols
WB protocol for EXOSC10 antibody 11178-1-APDownload protocol
IHC protocol for EXOSC10 antibody 11178-1-APDownload protocol
IF protocol for EXOSC10 antibody 11178-1-APDownload protocol
IP protocol for EXOSC10 antibody 11178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article
humanWB

Cell Rep

Human Telomerase RNA Processing and Quality Control.

Authors - Chi-Kang Tseng
  • KD Validated
View Article
mouseWB,RIP

Development

Post-transcription regulation by the exosome complex is required for cell survival and forebrain development by repressing P53 signaling.

Authors - Pauline Antonie Ulmke
  • KO Validated
View Article
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
View Article
humanWB

Life Sci Alliance

Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication

Authors - Tobias Moll
View Article
humanWB

Cancer Res

NAT10 drives cisplatin chemoresistance by enhancing ac4C-associated DNA repair in bladder cancer

Authors - Ruihui Xie
View Article