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验证数据展示

  • WB analysis using 31523-1-AP

    WB analysis using 31523-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31523-1-AP (EXOC8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31523-1-AP

    IF Staining of A431 using 31523-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using EXOC8 antibody (31523-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

EXOC8 Polyclonal antibody

EXOC8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31523-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

SEC84, Exo84p, EXO84

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuH-7 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31523-1-AP targets EXOC8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EXOC8 fusion protein Ag33300 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 exocyst complex component 8
别名 SEC84, Exo84p, EXO84
计算分子量 725 aa, 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC035763
基因名称 EXOC8
Gene ID (NCBI) 149371
RRIDAB_3670020
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8IYI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXOC8 is a subunit of the exocyst complex involved in docking exocytic vesicles with fusion sites on the plasma membrane. Truncated EXOC8 protein leads to the improper assembly of the exocyst complex, thus resulting in the accumulation of neurotransmitters and other excretory vesicles in the cell. It is found to play an essential role in normal cortical development and its perturbation causes complex brain disorders. EXOC8 mutations are linked to neurodevelopmental disorders with microcephaly, seizures, and brain atrophy (PMID: 35460391, PMID: 36344539).

实验方案

Product Specific Protocols
WB protocol for EXOC8 antibody 31523-1-APDownload protocol
IF protocol for EXOC8 antibody 31523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols