- Featured Product
- KD/KO Validated
EXOC1 Polyclonal antibody
EXOC1 Polyclonal Antibody for IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | rat brain tissue, human brain tissue, mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDA-MB-231 cells, human placenta tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11690-1-AP targets EXOC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF-P, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | EXOC1 fusion protein Ag2303 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | exocyst complex component 1 |
Synonyms | BM 102, EXOC1, exocyst complex component 1, Exocyst complex component Sec3, SEC3, SEC3L1, SEC3P |
Calculated Molecular Weight | 894 aa, 102 kDa |
Observed Molecular Weight | 102 kDa |
GenBank Accession Number | BC020650 |
Gene Symbol | EXOC1 |
Gene ID (NCBI) | 55763 |
RRID | AB_2231500 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NV70 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for EXOC1 antibody 11690-1-AP | Download protocol |
IHC protocol for EXOC1 antibody 11690-1-AP | Download protocol |
IF protocol for EXOC1 antibody 11690-1-AP | Download protocol |
IP protocol for EXOC1 antibody 11690-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Nanotechnol Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.
| ||
J Cell Biol The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells. | ||
Elife EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. | ||
PLoS Pathog Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape. | ||
Retrovirology Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation. |