验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, Jurkat cells, HEK-293 cells, HeLa cells, Raji cells |
| Positive IP detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16253-1-AP targets EXO1/Exonuclease 1 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9253 Product name: Recombinant human EXO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 496-846 aa of BC007491 Sequence: AVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREGACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLCLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | exonuclease 1 |
| 别名 | EXO1, EC:3.1.-.-, EXOI, Exonuclease 1, Exonuclease I |
| 计算分子量 | 94 kDa |
| 观测分子量 | 115-120 kDa |
| GenBank蛋白编号 | BC007491 |
| 基因名称 | EXO1 |
| Gene ID (NCBI) | 9156 |
| RRID | AB_2278140 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UQ84 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for EXO1/Exonuclease 1 antibody 16253-1-AP | Download protocol |
| WB protocol for EXO1/Exonuclease 1 antibody 16253-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling | ||
Nucleic Acids Res BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL | ||
Nucleic Acids Res DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway. | ||
Nucleic Acids Res Human HELQ regulates DNA end resection at DNA double-strand breaks and stalled replication forks
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