验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, RT-4 cells, U-251 cells, rat brain tissue, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32934-1-AP targets EVI5L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35996 Product name: Recombinant human EVI5L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 421-620 aa of BC014111 Sequence: VVRQQCSSAAEDLQKAQSTIRQLQEQQENPRLTEDFVSHLETELEQSRLRETETLGALREMQDKVLDMEKRNSSLPDENNVAQLQEELKALKVREGQAVASTRELKLQLQELSDTWQAHLARGGRWKESPRKLVVGELQDELMSVRLREAQALAEGRELRQRVVELETQDHIHRNLLNRVEAERAALQEKLQYLAAQNKG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ecotropic viral integration site 5-like |
| 别名 | Ecotropic viral integration site 5-like protein, EVI5-like protein |
| 观测分子量 | 91,92 kDa |
| GenBank蛋白编号 | BC014111 |
| 基因名称 | EVI5L |
| Gene ID (NCBI) | 115704 |
| RRID | AB_3742796 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96CN4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Evi5l (Ecotropic Viral Integration Site 5-Like), encodes a RabGAP that is known to block the formation of cilia when overexpressed (PMID: 26136363). EVI5L has ubiquitously low expression but is slightly higher in the testis and fetal brain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EVI5L antibody 32934-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
