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验证数据展示

  • WB analysis using 85943-1-RR

    WB analysis using 85943-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85943-1-RR (EVI2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 85943-1-RR

    IF Staining of THP-1 using 85943-1-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using EVI2B antibody (85943-1-RR, Clone: 250344C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EVI2B Recombinant antibody

EVI2B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85943-1-RR

产品说明书

CloneNo. 250344C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CD361, D17S376, EVDB, EVI 2B, EVI-2B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, Ramos cells, THP-1 cells, HL-60 cells
Positive IF/ICC detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy EVI2B Ready-To-Use IHC Kit. EVI2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85943-1-RR targets EVI2B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 EVI2B fusion protein Eg3709 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ecotropic viral integration site 2B
别名 CD361, D17S376, EVDB, EVI 2B, EVI-2B
计算分子量 448 aa, 49 kDa
观测分子量60 kDa
GenBank蛋白编号BC005926
基因名称 EVI2B
Gene ID (NCBI) 2124
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP34910
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ecotropic viral integration site 2B protein (EVI2B) is a type I transmembrane protein embedded within intron 27b of the neurofibromatosis type 1 (NF1) gene. It is transcribed in the opposite direction to the NF1 gene (PMID: 37584733). EVI2B is a downstream target of CCAAT/enhancer binding protein alpha (C/EBPα), which is crucial for myeloid differentiation and the function of hematopoietic progenitors (PMID: 36830696). This protein is highly expressed in mature granulocytes and is involved in the regulation of immune cell maturation (PMID: 28186500).

实验方案

Product Specific Protocols
WB protocol for EVI2B antibody 85943-1-RRDownload protocol
IF protocol for EVI2B antibody 85943-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols