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验证数据展示

  • WB analysis of MKN-45 using 60413-1-Ig

    WB analysis of MKN-45 using 60413-1-Ig

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60413-1-Ig

    WB analysis using 60413-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

EVI1 Monoclonal antibody

EVI1 Monoclonal Antibody for WB, ELISA
Cat No. 60413-1-Ig

产品说明书

CloneNo. 1A12A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367, AML1 EVI 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMKN-45 cells, Calu-3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy EVI1 Ready-To-Use IHC Kit. EVI1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60413-1-Ig targets EVI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EVI1 fusion protein Ag34143 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ecotropic viral integration site 1
别名 MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367, AML1 EVI 1
计算分子量 138 kDa
观测分子量140-150 kDa
GenBank蛋白编号NM_001105077
基因名称 EVI1
Gene ID (NCBI) 2122
RRIDAB_3670256
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ03112
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI1 antibody 60413-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols