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EVI1 Polyclonal antibody

EVI1 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 23201-1-AP

Print datasheet

Synonyms

AML1 EVI 1, EVI 1, EVI1, MDS1 EVI1, MECOM, PRDM3

验证数据展示

  • WB analysis of SKOV-3 using 23201-1-AP

    WB analysis of SKOV-3 using 23201-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 23201-1-AP (EVI1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 23201-1-AP

    IHC staining of human thyroid cancer using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IHC staining of human thyroid cancer using 23201-1-AP

    IHC staining of human thyroid cancer using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IHC staining of human urothelial carcinoma using 23201-1-AP

    IHC staining of human urothelial carcinoma using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IHC staining of human urothelial carcinoma using 23201-1-AP

    IHC staining of human urothelial carcinoma using 23201-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 23201-1-AP (EVI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EVI1 antibody included
  • IF Staining of SKOV-3 using 23201-1-AP

    IF Staining of SKOV-3 using 23201-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using EVI1 antibody (23201-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSKOV-3 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy EVI1 Ready-To-Use IHC Kit. EVI1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23201-1-AP targets EVI1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen EVI1 fusion protein Ag19603 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ecotropic viral integration site 1
Synonyms AML1 EVI 1, EVI 1, EVI1, MDS1 EVI1, MECOM, PRDM3
Calculated Molecular Weight 1239 aa, 139 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberBC130520
Gene Symbol EVI1
Gene ID (NCBI) 2122
RRIDAB_2879230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ03112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EVI1 antibody 23201-1-APDownload protocol
IHC protocol for EVI1 antibody 23201-1-APDownload protocol
IF protocol for EVI1 antibody 23201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Sci Adv

Predicting master transcription factors from pan-cancer expression data.

Authors - Jessica Reddy
View Article
humanCoIP

Oncol Rep

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis

Authors - Zhitao Chen
View Article