验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13182-1-AP targets ETV6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3852 Product name: Recombinant human ETV6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC043399 Sequence: MSETPAQCSIKQERISYTPPESPVPSYASSTPLHVPVPRALRMEEDSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQPEVILHQNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIRRLSPAERAQGPRPHQENNHQESYPLSVSPMENNHCPASSESHPKPSSPRQESTRVIQLMPSPIMHPLILNPRHSVDFKQSRLSEDGLHREGKPINLSHREDLAYMNHIMVSVSPPEEHAMPIGRIADCRLLWDYVYQLLSDSRYE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ets variant 6 |
| 别名 | ETS related protein Tel1, ETS translocation variant 6, ets variant 6, ETV6, TEL, TEL/ABL, TEL1, Transcription factor ETV6 |
| 计算分子量 | 452 aa, 53 kDa |
| 观测分子量 | 57-65 kDa |
| GenBank蛋白编号 | BC043399 |
| 基因名称 | ETV6 |
| Gene ID (NCBI) | 2120 |
| RRID | AB_2246688 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41212 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ETV6, the E26 transformation-specific (ETS) family transcription repressor and tumor suppressor variant 6 gene, resides on chromosome 12p13, encodes a 57 kDa ETS family transcription factor with 3 highly conserved functional domains(PMID: 32841218). ETV6 is a well-studied hematopoietic transcription factor reported to play a key functional role in hematopoiesis, megakaryopoiesis, cell growth, and differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETV6 antibody 13182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
