验证数据展示
经过测试的应用
| Positive WB detected in | T-47D cells, HeLa cells, PC-3 cells, DU 145 cells, LNCaP cells |
Planning an IHC experiment? We recommend our IHCeasy ETV5 Ready-To-Use IHC Kit. ETV5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| ChIP | See 4 publications below |
产品信息
66657-1-Ig targets ETV5 in WB, chIP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, chIP |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ETV5 fusion protein Ag3708 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ets variant 5 |
| 别名 | ERM, Ets related protein ERM, ETS translocation variant 5, ets variant 5, ETV5 |
| 计算分子量 | 510 aa, 58 kDa |
| 观测分子量 | 58-60 kDa |
| GenBank蛋白编号 | BC007333 |
| 基因名称 | ETV5 |
| Gene ID (NCBI) | 2119 |
| RRID | AB_2882014 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P41161 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ETS variant gene 5(ETV5) belongs to the ETS oncogene family that shares a conserved peptide, ETS domain, which mediates sequence-specific DNA binding. ETV5 is a transcription factor and is required for spermatogonial stem cell self-renewal. ETV5 can be negatively regulated by COP1, a tumor suppressor. Also it has a role in branching morphogenesis in the developing kidney
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ETV5 antibody 66657-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Etv transcription factors functionally diverge from their upstream FGF signaling in lens development. | ||
PLoS Genet FGF-induced Pea3 transcription factors program the genetic landscape for cell fate determination. | ||
Cardiovasc Toxicol ETV5-Mediated Transcriptional Repression of DDIT4 Blocks Macrophage Pro-Inflammatory Activation in Diabetic Atherosclerosis | ||
J Ovarian Res ANGPTL4 functions as an oncogene through regulation of the ETV5/CDH5/AKT/MMP9 axis to promote angiogenesis in ovarian cancer |