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验证数据展示

  • WB analysis of K-562 using 83891-6-RR

    WB analysis of K-562 using 83891-6-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83891-6-RR (ETV4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 83891-6-RR

    IHC staining of human colon cancer using 83891-6-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83891-6-RR (ETV4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 83891-6-RR

    IHC staining of human stomach cancer using 83891-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83891-6-RR (ETV4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 83891-6-RR

    IF Staining of A431 using 83891-6-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETV4 antibody (83891-6-RR, Clone: 241042E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of U2OS using 83891-6-RR

    FC experiment of U2OS using 83891-6-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug ETV4 Recombinant antibody (83891-6-RR, Clone:241042E10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83891-6-RR

    Affinity and Kinetic Characterization of 83891-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83891-6-RR against Human ETV4 were performed. The affinity constant is 0.157 nM.

ETV4 Recombinant monoclonal antibody

ETV4 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83891-6-RR

产品说明书

CloneNo. 241042E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

PEA3, ETS translocation variant 4, E1A-F, E1AF, Adenovirus E1A enhancer-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83891-6-RR targets ETV4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0984

Product name: Recombinant human ETV4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-207 aa of BC007242

Sequence: MYLHTEGFSGPSPGDGAMGYGYEKPLRPFPDDVCVVPEKFEGDIKQEGVGAFREGPPYQRRGALQLWQFLVALLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPFGPKGGYSY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ets variant 4
别名 PEA3, ETS translocation variant 4, E1A-F, E1AF, Adenovirus E1A enhancer-binding protein
计算分子量 54 kDa
观测分子量 61-65 kDa
GenBank蛋白编号BC007242
基因名称 ETV4
Gene ID (NCBI) 2118
RRIDAB_3671474
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP43268
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. The calculated molecular weight of ETV4 protein is 54 kDa, but the phosphorylated ETV4 is a 54-61 kDa protein. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene.

实验方案

Product Specific Protocols
WB protocol for ETV4 antibody 83891-6-RRDownload protocol
IHC protocol for ETV4 antibody 83891-6-RRDownload protocol
IF protocol for ETV4 antibody 83891-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols