验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11721-1-AP targets ETV3 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag2301 Product name: Recombinant human ETV3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-143 aa of BC022868 Sequence: MKAGCSIVEKPEGGGGYQFPDWAYKTESSPGSRQIQLWHFILELLQKEEFRHVIAWQQGEYGEFVIKDPDEVARLWGRRKCKPQMNYDKLSRALRYYYNKRILHKTKGKRFTYKFNFNKLVMPNYPFINIRSSGKIQTLLVGN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ets variant 3 |
| 别名 | ets variant 3, ETV 3, PE-1 |
| 计算分子量 | 512 aa, 57 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC022868 |
| 基因名称 | ETV3 |
| Gene ID (NCBI) | 2117 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41162 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ETV3 is a transcriptional repressor that belongs to the ETS family of transcription factors, which bind a common DNA sequence core motif (GGAA) via their ETS domains. ETV3 is preferentially expressed in mature DC including tissue-derived migratory DC (migDC) and facilitates their homeostatic maturation and CCR7-dependent migration. Phosphorylation of ETV3 regulated transcription by preventing its binding to DNA at promoters for several thousand genes, including some involved in negative feedback regulation of itself and of upstream signals (PMID: 36543959, 41678619). Western and IP analysis detected the specific band with an apparent MW of 57 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for ETV3 antibody 11721-1-AP | Download protocol |
| WB protocol for ETV3 antibody 11721-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
