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验证数据展示

  • WB analysis using 12262-1-AP (same clone as 12262-1-PBS)

    WB analysis using 12262-1-AP (same clone as 12262-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12262-1-AP (ETFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12262-1-AP (same clone as 12262-1-PBS)

    IHC staining of human breast cancer using 12262-1-AP (same clone as 12262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12262-1-AP (ETFA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ETFA antibody included
  • IF Staining of HeLa using 12262-1-AP (same clone as 12262-1-PBS)

    IF Staining of HeLa using 12262-1-AP (same clone as 12262-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ETFA antibody (12262-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red). This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 12262-1-AP (same clone as 12262-1-PBS)

    FC experiment of MCF-7 using 12262-1-AP (same clone as 12262-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug ETFA Polyclonal antibody (12262-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12262-1-PBS in a different storage buffer formulation.

ETFA Polyclonal antibody, PBS Only

ETFA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 12262-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Alpha ETF, Alpha-ETF, EMA, GA2, MADD

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ETFA Ready-To-Use IHC Kit. ETFA primary antibody included.

产品信息

12262-1-PBS targets ETFA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2931

Product name: Recombinant human ETFA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-333 aa of BC015526

Sequence: MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 electron-transfer-flavoprotein, alpha polypeptide
别名 Alpha ETF, Alpha-ETF, EMA, GA2, MADD
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC015526
基因名称 ETFA
Gene ID (NCBI) 2108
RRIDAB_2100540
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13804
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.

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