验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HSC-T6 cells, MOLT-4 cells, Jurkat cells, K-562 cells, THP-1 cells |
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy ESYT1 Ready-To-Use IHC Kit. ESYT1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67688-1-Ig targets ESYT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag16840 Product name: Recombinant human FAM62A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 755-1104 aa of BC004998 Sequence: DVPSGRLHLRLERLTPRPTAAELEEVLQVNSLIQTQKSAELAAALLSIYMERAEDLPLRKGTKHLSPYATLTVGDSSHKTKTISQTSAPVWDESASFLIRKPHTESLELQVRGEGTGVLGSLSLPLSELLVADQLCLDRWFTLSSGQGQVLLRAQLGILVSQHSGVEAHSHSYSHSSSSLSEEPELSGGPPHITSSAPELRQRLTHVDSPLEAPAGPLGQVKLTLWYYSEERKLVSIVHGCRSLRQNGRDPPDPYVSLLLLPDKNRGTKRRTSQKKRTLSPEFNERFEWELPLDEAQRRKLDVSVKSNSSFMSRERELLGKVQLDLAETDLSQGVARWYDLMDNKDKGSS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | family with sequence similarity 62 (C2 domain containing), member A |
| 别名 | E Syt1, ESYT1, E-Syt1, Extended synaptotagmin 1, Extended synaptotagmin-1, FAM62A, KIAA0747, MBC2 |
| 计算分子量 | 1104 aa, 123 kDa |
| 观测分子量 | 123 kDa |
| GenBank蛋白编号 | BC004998 |
| 基因名称 | ESYT1 |
| Gene ID (NCBI) | 23344 |
| RRID | AB_2882881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BSJ8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ESYT1 antibody 67688-1-Ig | Download protocol |
| WB protocol for ESYT1 antibody 67688-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair
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Nat Cell Biol Sphingosine-1-phosphate signalling activates E-Syt1 to facilitate HDL-derived cholesterol transport
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