验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HEK-293 cells, LNCaP cells, PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33574-1-AP targets ESS2/DGCR14 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37915 Product name: Recombinant human ESS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-250 aa of NM_022719 Sequence: METPGASASSLLLPAASRPPRKREAGEAGAATSKQRVLDEEEYIEGLQTVIQRDFFPDVEKLQAQKEYLEAEENGDLERMRQIAIKFGSALGKMSREPPPPYVTPATFETPEVHAGTGVVGNKPRPRGRGLEDGEAGEEEEKEPLPSLDVFLSRYTSEDNASFQEIMEVAKERSRARHAWLYQAEEEFEKRQKDNLELPSAEHQAIESSQASVETWKYKAKNSLMYYPEGVPDEEQLFKKPRQVVHKNTR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DiGeorge syndrome critical region gene 14 |
| 别名 | DGCR14, ESS2, DGCR13, DGSH, DGS-H |
| 计算分子量 | 476aa, 53 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | NM_022719 |
| 基因名称 | DGCR14 |
| Gene ID (NCBI) | 8220 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96DF8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ESS2 splicing factor homolog (ESS2), also named DiGeorge-syndrome critical region 14 (DGCR14), acts as a transcriptional co-activator for retinoic acid receptor-related orphan nuclear receptor gamma/gamma-t (Rorγ/γt). Ess2 is a nuclear protein with a coiled-coil domain [6] that is conserved from yeasts to humans. ESS2 is a member of the deletion gene cluster in the 22q11.2 deletion syndrome (22q11.2DS, also known as DiGeorge syndrome or CATCH 22 syndrome). The syndrome is characterized by varying defects of the heart, thymus and parathyroid glands.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ESS2/DGCR14 antibody 33574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
